1. Aleksandra Šmitran, University of Banja Luka, Medical Faculty,
Republic of Srpska, Bosnia and Herzegovina
2. Dragana Gajic, Republic of Srpska, Bosnia and Herzegovina
3. Ljiljana Bozic, Republic of Srpska, Bosnia and Herzegovina
4. Dijana Jelić, Medicinski fakultet Banja Luka , Republic of Srpska, Bosnia and Herzegovina
Abstract: Novel antimicrobial substances are among the most exploring in scientific circles nowadays. Nanocomposites proved to be one of the promising candidates. Novel research based on nanocomposites goes in the direction of synthesizing hybrid materials based on inorganic matrix of layered structure. Clays possess such specific layered structures and their structure can be modified. In this research pyrophyllite clay was in focus and was modified with silver, well known antimicrobial metal. The main emphasis in this study was on antimicrobial efficiency of nanocomposite (pyrophyllite clay based) against planktonic (individual) isolates and against biofilm (community bacterial forms of living). Antibacterial activity against planktonic isolates of E. coli ATCC 8739 and S. aureus ATCC 25923 was detected with bujon microdilution method. Microtiter plates were inoculated with 100 μl of bacterial inoculum and treated with 20 and 40 mg of pyrophyllite clay/Ag material during overnight incubation with shaking at 37 °C. Antibiofilm testing with 10 mg of nanocomposite was performed in microtiter plate against biofilm producing clinical isolates of Acinetobacter baumannii and Pseudomonas aeruginosa. In conclusion, nanocomposites of pyrophyllite clay/Ag have excellent in vitro activity against all living forms of bacteria which could have enormous potential for wide practical usage of these substances.
Authors are thankful to the Ministry for Scientific and Technological Development, Higher Education and Information Society of Republic of Srpska for supporting the study through the project No. 19.032/961-78/19.
Key wordstestttt :
SIMPOZIJUM B - Biomaterijali i nanomedicina