PROLONGED EXPOSURE TO MESOPOROUS SILICA DECREASE CELL VIABILITY IN VITRO

1. Ana Valenta Šobot, Serbia
2. Jelena Filipović Tričković, Serbia
3. Aleksandra Šaponjić, Serbia
4. Ana Grce, Serbia
5. Maja Kokunešoski, Serbia

Ordered mesoporous silica SBA-15 structure with high specific surface area enables large pharmaceutical adsorption capacities and promotes its application as a carrier in drug formulations of prolonged-release. The aim of the study was to estimate SBA-15 time and concentration impact on human peripheral blood mononuclear cells viability, as well as cellular morphology and DNA fragmentation in vitro. SBA-15 mesoporous silica treatment impact on cell viability was monitored at 24, 48 and 72 h time points by TB assay, while at the end of the treatment DNA fragmentation was assessed by colorimetric assay and cellular morphology by dual TMRE/DAPI fluorescent staining. SBA-15 cytotoxic potential rises dependently on time and concentration exposure. After 72 h, all tested concentrations were cytotoxic and displayed elevated DNA fragmentation corresponding to a high level of apoptotic and necrotic cells, as shown by dual fluorescent staining. Short term exposure to SBA-15 material or chemical modification that could influence its physicochemical properties could be a way to lower its toxicity.

Ključne reči: SBA-15; cytotoxicity; DNA fragmentation; TMRE staining

Tematska oblast: SIMPOZIJUM B - Biomaterijali i nanomedicina

Datum: 30.06.2021.

Contemporary Materials 2021 - Savremeni Materijali


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