Ordered mesoporous silica SBA-15 structure with high specific surface area enables large pharmaceutical adsorption capacities and promotes its application as a carrier in drug formulations of prolonged-release. The aim of the study was to estimate SBA-15 time and concentration impact on human peripheral blood mononuclear cells viability, as well as cellular morphology and DNA fragmentation in vitro. SBA-15 mesoporous silica treatment impact on cell viability was monitored at 24, 48 and 72 h time points by TB assay, while at the end of the treatment DNA fragmentation was assessed by colorimetric assay and cellular morphology by dual TMRE/DAPI fluorescent staining. SBA-15 cytotoxic potential rises dependently on time and concentration exposure. After 72 h, all tested concentrations were cytotoxic and displayed elevated DNA fragmentation corresponding to a high level of apoptotic and necrotic cells, as shown by dual fluorescent staining. Short term exposure to SBA-15 material or chemical modification that could influence its physicochemical properties could be a way to lower its toxicity.
Ključne reči :
SIMPOZIJUM B - Biomaterijali i nanomedicina