1. Milan Mataruga, Faculty of Forestry, University of Banja Luka,
Republic of Srpska, Bosnia and Herzegovina
2. Vanja Daničić, Faculty of Forestry, University of Banja Luka, Republic of Srpska, Bosnia and Herzegovina
3. Barbara Fussi, Bayerisches Amt für forstliche Saat- und Pflanzenzucht, Germany
4. Branislav Cvjetković, Faculty of Forestry, University of Banja Luka, Republic of Srpska, Bosnia and Herzegovina
5. Vasilije Isajev, Serbia
Serbian spruce (Picea omorika Pančić/Purkyne) is an endemic coniferous species in the Drina River valley in eastern Bosnia and Herzegovina and western Serbia with an extremely small and scattered natural distribution. Serbian spruce has been a legally protected species in Serbia and Bosnia and Herzegovina since 1964 and is listed in the IUCN Red List of Threatened Species owing to its limited natural range (Conifer Specialists Group 1998). Taking into account the specificity of geographical distribution, degree of protection, the number of natural populations, this species has been studied for a long time from the aspect of genetic diversity.
Altogether 689 individuals from of P. omorika 14 populations (29 to 67 individuals per population) were sampled in 2016. DNA was extracted from needles using the CTAB method following DOYLE AND DOYLE (1990). Before extraction, needles were lyophilized. Molecular tools comprised 10 nuclear microsatellites (WS00716.F13, WS0092.M15, WS0022.B15, WS0073.H08, WS00111.K13, Pa51, Pa47, WS0092.A19, WS0046.M11, WS0032.M17_ schwarz). The obtained data was analyzed using the statistical programs GenAlEx 6.501 (PEAKALL AND SMOUSE 2012), Micro-Checker 2.2.3. (VAN OSTERHOUT ET AL. 2004) and Structure 2.3.4. (PRITCHARD ET AL. 2000).
The number of detected alleles for each locus varied between 2 and 24, and mean number of alleles per locus varied between 2,90 (Panjak) and 5,00 (Veliki Stolac). Similar large differences between populations were found for the mean values of rare alleles varying from 2,89 (Panjak) to 4.59 (Veliki Stolac). Private alleles were found in five out of 14 analyzed populations; Viagor and Krušev do with a mean of 0,2 and Veliki Stolac, Vidikovac, Radomišlja with a mean of 0.1. Null alleles were detected only for one locus (WS00111.K13) in the population Baba-Starogorska stijena. The mean number of effective alleles varied between 2.10 (Radomišlja) and 3.03 (Suvi do). The observed heterozygosity ranged from 0.395 (Vidikovac) to 0,540 (Grad). Values for expected heterozygosity varied between 0.394 (Vidikovac) and 0,493 (Suvi do and Grad).
The UPGMA analysis revealed different clustering pattern between populations, being the grouping according to geographic distances. Analysis of nSSR data using Structure software revealed 9 clusters. The statistical analysis of the nSSR results through PCoA based on Nei’s distance revealed a clear genetic differentiation of the Radomišlja population compared to the remaining populations. Interpretation of the results in the view of gene conservation and seed stand selection will be presented.
Šumarska genetika, sjemenarstvo, rasadničarstvo i osnivanje šuma