1. Savka Janković, Prirodno-matematički fakultet Banja Luka,
Republic of Srpska, Bosnia and Herzegovina
2. Aleksandra Šmitran, University of Banja Luka, Faculty of Medicine, Save Mrkalja 14, Republic of Srpska, Bosnia and Herzegovina
3. Sanja Šehovac, Prirodno-matematički fakultet Banja Luka, Republic of Srpska, Bosnia and Herzegovina
4. Dragana Milisavić, Prirodno-matematički fakultet Banja Luka, Republic of Srpska, Bosnia and Herzegovina
5. Mladena Malinović, University of Banja Luka, Faculty od Natural Sciences and Mathematics, Chemistry Department, Mladena, Republic of Srpska, Bosnia and Herzegovina
6. Dijana Jelić, Medicinski fakultet Banja Luka , Republic of Srpska, Bosnia and Herzegovina
To be used for biomedical purposes, the nanoparticle (NP) must meet the following criteria: low-risk toxicological profile, long-term physical stability and high adsorption capacity power. Precise and detailed control of the surface of the NP, or knowledge of surface chemistry, is very important when the NP is connected with biology. Thanks to the better ratio between the volume and the surface in the NP in relation to the larger particles, as well as the better magnetic properties, the nanoparticle application for biomedical purposes has become multifunctional. The chemical composition of the nanoparticle, its structure, size, and coating of the nanoparticle depend primarily on the synthesis method. The method of synthesis of the nanoparticle itself controls the physicochemical and biochemical properties of the NP, and thus its application. The green chemistry method is currently very effective because it is an ecologically acceptable method that uses biological molecules from plant species (phytosynthesis) in the form of extracts for the purposes of reduction processes. Most plant species contain flavonoids, phenols, alcohols, and proteins that can produce metal nanoparticles from metal salts by reduction process. Here, Cu dopped zink oxide nanoparticles (ZnO NP) were synthesized by using black and green tea, vitamine C and trisodium citrate as a reduction agents. Antimicrobial and photocatalytical properties were tested. The antimicrobial sensitivity of the dopped synthesized ZnO NP on the isolates Acinetobacter baumannii and methicillin resistant Staphylococcus aureus (MRSA) was performed by the diffusion method on the Muller-Hinton substrate. ZnO NP with all four reduction agents showed antimicrobial activity toward MRSA with inhibition zone 10 mm, while only dopped ZnO NP synthesized with green and black tea showed antimicrobial sensitivity for Acinetobacter baumannii with average value of inhibition zone around 8 mm. Photocatalytical activity was more pronaunced in case of undoped ZnO nanoparticles.