1. Teodora Tomović, Prirodno-matematički fakultet Banja Luka,
Republic of Srpska, Bosnia and Herzegovina
2. Dijana Jelić, Univerzitet u Banjoj Luci, Prirodno-matematički fakultet, Studijski program Hemija,
Republic of Srpska, Bosnia and Herzegovina
3. Aleksandra Šmitran, University of Banja Luka, Medical Faculty,
Republic of Srpska, Bosnia and Herzegovina
Selenium, already well known as an essential mineral, shows also good antimicrobial activity against different pathogens. Selenium nanoparticles (Se NPs), compared with selenium inorganic and organic compounds, emerged as a promising agent for biomedical uses due to their low toxicity, degradability and high bioavailability. When it comes to nanoparticles, their ability for aggregation is quite challenging. In order to avoid this unfavorable occurence, one uses surfactant-coated nanoparticles methodology. Basically, nanoparticles are surrounded and covered by the surfactant which allows NPs to be more stable. In this research two cationic surfactants were used: alkyldimethylbenzylammonium chloride (ADBAC) and cetyltrimethylammonium bromide (CTAB) Cationic surfactant contains alkylamine or quaternary ammonium salts in their hydrophilic groups and can be easily adsorbed onto negatively charged interfaces such as fibers, keratin or bacterial proteins. To investigate influence of two different surfactant-coatings on selenium NPs and their antimicrobial properties, three carbapenemase-resistant clinical isolates of Acinteobacter baumannii (CRAB) were incorporated in this study. Minimum inhibitory concentrations (MIC) of Se NPs were investigated by broth microdilution method in microtiter plates filled with 100µl of doubled-strength Muller Hinton broth, 50 µl of bacterial inoculum (0.5 McFarlands turbidity) and 50 µl of Se NPs in final concentration ranging from 2.5-0.003 mg/ml. CTAB-coated SeNPs dissolved in water showed constant antimicrobial activity amounted in two consecutive concentration of 0.07-0.15 mg/ml against all three tested isolates. Given that, these NPs were dissolved in water, this is an extremely good result when it comes to multiresistant CRAB isolates. ADBAC-coated SeNPs dissolved in DMSO showed antimicrobial activity in three distinctive concentrations, 0.015, 0.06 and 0.125 mg/ml. Additionally, the antimicrobial activity of the DMSO solvent was also analyzed and noticed. CTAB-coated SeNPs showed better antimicrobial activity comparing to ADBAC-coated SeNPs. Our results showed that antimicrobial activity of ADBAC-coated SeNPs is due to both, SeNPs and DMSO solvent
Ključne reči :
Tematska oblast:
SIMPOZIJUM B - Biomaterijali i nanomedicina
Datum:
28.07.2022.
Contemporary Materials 2022 - Savremeni materijali
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